Recommendations from the manufacturers (depending on a few factors) are usually somewhere between 12 and 20ish uses (tightening to recommended torque) for a small steel nyloc nut, to guarantee that they stay on.
You can get away with more uses, but the manufacturers aren't sure the nut will meet end load/torque/retention targets.
FWIW, on my RC cars (and push bikes) i just keep using them until i decide its time to change. Probably 2 or 3 times the recommended number of uses. At work we tend to bin after a single use as its really not worth trying to work out how many times they've been used. Not when we probably pay less than a penny per nut.
Not dealt with aluminium nylocs, but i would expect about 2/3rds of the durability.
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